CTNNA1 Antikörper (pSer641)

Produktnummer ABIN401554

Produktdetails anti-CTNNA1 Antikörper

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Bindungsspezifität: pSer641

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTNNA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This antibody detects endogenous levels of Alpha-Catenin only when phosphorylated at Serine 641.

Aufreinigung: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around the phosphorylation site of Serine 641 (D-D-Sp-D-F) derived from Human Alpha-Catenin.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at-20 °C.

Details zu CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Andere Bezeichnung: Catenin alpha-1 / CTNNA1 (CTNNA1 Produkte)

Hintergrund: The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kDa) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR.Synonyms: Alpha Catenin, Alpha E-catenin, Cadherin-associated protein, NY-REN-13 antigen

Gen-ID: 1495

NCBI Accession: NP_001894

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location