CHEK1 Antikörper (pSer280)

Produktnummer ABIN401536

Produktdetails anti-CHEK1 Antikörper

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Bindungsspezifität: pSer280

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 280.

Aufreinigung: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 280 (V-T-Sp-G-G).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at-20 °C.

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: CHK1 (CHEK1 Produkte)

Synonyme: An08g10320 antikoerper, AO090003000441 antikoerper, CHK1 antikoerper, chk1 antikoerper, C85740 antikoerper, Chk1 antikoerper, rad27 antikoerper, id:ibd2720 antikoerper, zgc:56093 antikoerper, CHEK1 antikoerper, checkpoint kinase 1 antikoerper, serine/threonine-protein kinase chk1 antikoerper, serine/threonine-protein kinase Chk1 antikoerper, CAMK/CAMKL/CHK1 protein kinase Chk1 antikoerper, checkpoint kinase 1 S homeolog antikoerper, CHEK1 antikoerper, ANI_1_2436074 antikoerper, AOR_1_768154 antikoerper, PTRG_04183 antikoerper, SJAG_01680 antikoerper, PAAG_04978 antikoerper, MCYG_03290 antikoerper, VDBG_03742 antikoerper, chek1.S antikoerper, Chek1 antikoerper, chek1 antikoerper

Hintergrund: One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic dependent kinase Cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of Cdc2. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent 14-3-3 binding negatively regulated Cdc25C, thus preventing it from activating Cdc2.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Gen-ID: 1111

NCBI Accession: NP_001107593

UniProt: O14757

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur