ATR Antikörper (Internal Region)

Produktnummer ABIN401401

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ATR in WB und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus, Hund, Fisch, Affe, Ratte und Xenopus laevis.

Kurzübersicht für ATR Antikörper (Internal Region) (ABIN401401)

Target: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Reaktivität: Human, Maus, Hund, Fisch, Affe, Ratte, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-ATR Antikörper

Bindungsspezifität: Internal Region

Spezifität: This antibody is specific for ATR protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Monkey, Canine, Fish, Xenopus.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human ATR protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/15,000 - 1/70,000. Western Blot: 1/1,000 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.28 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu ATR

Target: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Andere Bezeichnung: ATR / FRP1

Hintergrund: Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNAPK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during Sphase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.Synonyms: Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, Serine/threonine-protein kinase ATR

Gen-ID: 545

NCBI Accession: NP_001175

UniProt: Q13535

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus