PCNA Antikörper (Internal Region)

Produktnummer ABIN401372

Der Kaninchen Polyklonal anti-PCNA Antikörper wird verwendet zum Nachweis von PCNA in Proben von Human, Maus, Ratte, Huhn, Affe, Hund, Rind (Kuh), Xenopus laevis und Fisch. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für PCNA Antikörper (Internal Region) (ABIN401372)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Hund, Rind (Kuh), Xenopus laevis, Fisch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PCNA Antikörper

Bindungsspezifität: Internal Region

Spezifität: This antibody is directed against PCNA protein.

Kreuzreaktivität (Details): Species reactivity (expected):Monkey, Canine, Mouse, Rat, Bovine, Chicken, Fish, Xenopus.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human PCNA protein

Anwendungsinformationen

Applikationshinweise: ELISA: 1/2,000 - 1/8,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry on Paraffin sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.62 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for up to one month or (in aliquots)at-20 °C for longer.

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand. PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision. PCNA is a useful marker for DNA synthesis and is highly conserved among most species.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

Gen-ID: 9606

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie