Der Kaninchen Polyklonal anti-CHEK1 Antikörper wird verwendet zum Nachweis von CHEK1 in Proben von Saccharomyces cerevisiae. Er wurde validiert für WB und EIA.
ELISA: 1/2,000 - 1/10,000. Western Blot: 1/200 - 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.73 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Target
CHEK1
(Checkpoint Kinase 1 (CHEK1))
Andere Bezeichnung
CHK1
Hintergrund
CHK1 (also known as serine/threonine-protein kinase CHK1 and checkpoint kinase 1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. The kinase controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit. CHK1 is localized within the nucleus.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1