PFKL Antikörper (C-Term)

Produktnummer ABIN392745

Dieses Anti-PFKL-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PFKL in WB und IHC (p). Geeignet für Human und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für PFKL Antikörper (C-Term) (ABIN392745)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PFKL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB03903

Produktdetails anti-PFKL Antikörper

Bindungsspezifität: AA 669-699, C-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PFKL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 669-699 amino acids from the C-terminal region of human PFKL.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-PFKL Antikörper (ABIN392745)

Mikawa, Maruyama, Okamoto, Nakagama, Lleonart, Tsusaka, Hori, Murakami, Izumi, Takaori-Kondo, Yokode, Peters, Beach, Kondoh: "Senescence-inducing stress promotes proteolysis of phosphoglycerate mutase via ubiquitin ligase Mdm2." in: The Journal of cell biology, Vol. 204, Issue 5, pp. 729-45, (2014) (PubMed).

Details zu PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Andere Bezeichnung: PFKL

Hintergrund: Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues, human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.

Molekulargewicht: 85018

Gen-ID: 5211

NCBI Accession: NP_002617

UniProt: P17858

Pathways: Negative Regulation of Hormone Secretion, Warburg Effekt