SPHK1 Antikörper (N-Term)

Produktnummer ABIN391347

Dieses Anti-SPHK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SPHK1 in WB und IHC (p). Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für SPHK1 Antikörper (N-Term) (ABIN391347)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPHK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB15394

Produktdetails anti-SPHK1 Antikörper

Bindungsspezifität: AA 59-89, N-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the N-terminal region of human SPHK1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:100. IHC-P: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Haltbarkeit: 6 months

Referenzen für anti-SPHK1 Antikörper (ABIN391347)

Kim, Park, Kim, DAgati, Lee: "Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury." in: Anesthesiology, Vol. 114, Issue 2, pp. 363-73, (2011) (PubMed).

Di, Kawamura, Gao, Tang, Berdyshev, Vogel, Zhao, Sharma, Bachmaier, Xu, Malik: "A novel function of sphingosine kinase 1 suppression of JNK activity in preventing inflammation and injury." in: The Journal of biological chemistry, Vol. 285, Issue 21, pp. 15848-57, (2010) (PubMed).

Details zu SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Andere Bezeichnung: SPHK1

Hintergrund: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

NCBI Accession: NP_001136073, NP_001136074, NP_068807, NP_892010

UniProt: Q9NYA1

Pathways: VEGF Signaling