SPHK1 Antikörper (AA 286-315)

Produktnummer ABIN391346

Der Kaninchen Polyklonal Anti-SPHK1-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, SPHK1 in Proben von Human, Maus und Ratte zu detektieren. Es sind 22+ Publikationen verfügbar.

Kurzübersicht für SPHK1 Antikörper (AA 286-315) (ABIN391346)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPHK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB03621

Produktdetails anti-SPHK1 Antikörper

Bindungsspezifität: AA 286-315

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000. WB: 1:1000. WB: 1:2000. IHC-P: 1:25. IHC-P: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-SPHK1 Antikörper (ABIN391346)

Furuya, Shimizu, Tamashiro, Iino, Bielawski, Chan, Pagano, Kawamori: "Sphingosine kinase 1 expression enhances colon tumor growth." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 120, (2018) (PubMed).

Song, Zhang, Chen, Xia, Qin, Waeber, Sheng: "Sphingosine kinase 2 activates autophagy and protects neurons against ischemic injury through interaction with Bcl-2 via its putative BH3 domain." in: Cell death & disease, Vol. 8, Issue 7, pp. e2912, (2018) (PubMed).

Zhu, You, Tan, Li, Qiu, Li, Huang, Zheng, Ren: "Overexpression of sphingosine kinase 1 is predictive of poor prognosis in human breast cancer." in: Oncology letters, Vol. 14, Issue 1, pp. 63-72, (2017) (PubMed).

Do, Kim, Kim, Lee, Do, Kim, Chae, Sohn: "Predictive Value of Sphingosine Kinase 1 Expression in Papillary Thyroid Carcinoma." in: Anticancer research, Vol. 37, Issue 10, pp. 5399-5405, (2017) (PubMed).

Gairhe, Joshi, Bastola, McLendon, Oka, Fagan, McMurtry: "Sphingosine-1-phosphate is involved in the occlusive arteriopathy of pulmonary arterial hypertension." in: Pulmonary circulation, Vol. 6, Issue 3, pp. 369-80, (2016) (PubMed).

Dick, Hengst, Fox, Colledge, Kale, Sung, Sharma, Amin, Loughran, Kester, Wang, Yun: "The apoptotic mechanism of action of the sphingosine kinase 1 selective inhibitor SKI-178 in human acute myeloid leukemia cell lines." in: The Journal of pharmacology and experimental therapeutics, Vol. 352, Issue 3, pp. 494-508, (2015) (PubMed).

Zheng, Wei, Wang, Xu, Xiao, Liu, Jia, Cheng: "Sphingosine kinase 1 mediates neuroinflammation following cerebral ischemia." in: Experimental neurology, (2015) (PubMed).

Matula, Collie-Duguid, Murray, Parikh, Grabsch, Tan, Lalwani, Garau, Ong, Bain, Smith, Urquhart, Bielawski, Finnegan, Petty: "Regulation of cellular sphingosine-1-phosphate by sphingosine kinase 1 and sphingosine-1-phopshate lyase determines chemotherapy resistance in gastroesophageal cancer." in: BMC cancer, Vol. 15, pp. 762, (2015) (PubMed).

Xiong, Wang, Guan, Wu, Xu, Wang, Rong, Huang, Huang, Liao, Fu, Yuan: "SphK1 confers resistance to apoptosis in gastric cancer cells by downregulating Bim via stimulating Akt/FoxO3a signaling." in: Oncology reports, Vol. 32, Issue 4, pp. 1369-73, (2014) (PubMed).

Altay, Suzuki, Hasegawa, Caner, Krafft, Fujii, Tang, Zhang: "Isoflurane attenuates blood-brain barrier disruption in ipsilateral hemisphere after subarachnoid hemorrhage in mice." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2513-6, (2012) (PubMed).

Fischer, Alliod, Martinier, Newcombe, Brana, Pouly: "Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions." in: PLoS ONE, Vol. 6, Issue 8, pp. e23905, (2011) (PubMed).

Song, Xiong, Li, Liao, Wang, Wu, Li: "Sphingosine kinase-1 enhances resistance to apoptosis through activation of PI3K/Akt/NF-?B pathway in human non-small cell lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 7, pp. 1839-49, (2011) (PubMed).

Kim, Park, Kim, DAgati, Lee: "Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury." in: Anesthesiology, Vol. 114, Issue 2, pp. 363-73, (2011) (PubMed).

Kim, Park, Kim, DAgati, Lee: "Isoflurane activates intestinal sphingosine kinase to protect against bilateral nephrectomy-induced liver and intestine dysfunction." in: American journal of physiology. Renal physiology, Vol. 300, Issue 1, pp. F167-76, (2011) (PubMed).

Nayak, Huo, Kwang, Pushparaj, Kumar, Ling, Dheen: "Sphingosine kinase 1 regulates the expression of proinflammatory cytokines and nitric oxide in activated microglia." in: Neuroscience, Vol. 166, Issue 1, pp. 132-44, (2010) (PubMed).

Liu, Zheng, Zhang, Wu, Xiong, Li, Song: "Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy." in: BMC cancer, Vol. 10, pp. 495, (2010) (PubMed).

Crousillac, Colonna, McMains, Dewey, Gleason: "Sphingosine-1-phosphate elicits receptor-dependent calcium signaling in retinal amacrine cells." in: Journal of neurophysiology, Vol. 102, Issue 6, pp. 3295-309, (2009) (PubMed).

Wadgaonkar, Patel, Grinkina, Romano, Liu, Zhao, Sammani, Garcia, Natarajan: "Differential regulation of sphingosine kinases 1 and 2 in lung injury." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 296, Issue 4, pp. L603-13, (2009) (PubMed).

Li, Yu, Xia, Zhang, Weng, Zheng, Kong, Hu, Zeng, Zeng, Li, Li, Song: "Sphingosine kinase 1 is associated with gastric cancer progression and poor survival of patients." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 4, pp. 1393-9, (2009) (PubMed).

Li, Guan, Gong, Song, Zhang, Wu, Yuan, Zheng, Huang, Li: "Clinical significance of sphingosine kinase-1 expression in human astrocytomas progression and overall patient survival." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 21, pp. 6996-7003, (2008) (PubMed).

Details zu SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Andere Bezeichnung: SPHK1

Hintergrund: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Gen-ID: 8877

NCBI Accession: NP_001136073, NP_001136074, NP_068807, NP_892010

UniProt: Q9NYA1

Pathways: VEGF Signaling