CRY2 Antikörper (C-Term)

Produktnummer ABIN390080

Der Kaninchen Polyklonal Anti-CRY2-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, CRY2 in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CRY2 Antikörper (C-Term) (ABIN390080)

Target: CRY2 (Cryptochrome 2 (Photolyase-Like) (CRY2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CRY2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB1846

Produktdetails anti-CRY2 Antikörper

Bindungsspezifität: AA 564-593, C-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Cry2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Cry2.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-CRY2 Antikörper (ABIN390080)

Wang, Wang, Liu, Liu, Tay, Walsh, Yang, Wu: "CRISPR/Cas9 mediated genome editing of Helicoverpa armigera with mutations of an ABC transporter gene HaABCA2 confers resistance to Bacillus thuringiensis Cry2A toxins." in: Insect biochemistry and molecular biology, Vol. 87, pp. 147-153, (2017) (PubMed).

Details zu CRY2

Target: CRY2 (Cryptochrome 2 (Photolyase-Like) (CRY2))

Andere Bezeichnung: Cry2

Hintergrund: Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central ?gears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function, in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Molekulargewicht: 66947

Gen-ID: 1408

NCBI Accession: NP_001120929, NP_066940

UniProt: Q49AN0

Pathways: Response to Water Deprivation, Protein targeting to Nucleus