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CHEK1 Antikörper (pSer317)

Name: CHEK1 Antikörper (pSer317)
SKU: ABIN389543
Price: 434.77 EUR
Availability: InStock

Der Kaninchen Polyklonal Anti-CHEK1-Antikörper wurde für WB, IHC (p) und DB validiert. Er ist geeignet, CHEK1 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

The anti-Phospho-CHK1- Pab (ABIN391324 and ABIN2841354) is used in Western blot for detection in, from left to right, Jurkat, , Hela, and HL60 tissue lysates. (CHEK1 Antikörper (pSer317))

Dot blot analysis of anti-Phospho-CHK1- Antibody (ABIN389543 and ABIN2839588) on nitrocellulose membrane. (CHEK1 Antikörper (pSer317))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (CHEK1 Antikörper (pSer317))

IHC (p)

3 Abbildungen

(1 reference)

Produktnummer ABIN389543

Kurzübersicht für CHEK1 Antikörper (pSer317) (ABIN389543)

Target

Alle CHEK1 Antikörper anzeigen

CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität

Human

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser CHEK1 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Klon

RB7845-RB18425

Discover our top product CHEK1 Primärantikörper

Bindungsspezifität
35

28

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16

9

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pSer317

Aufreinigung

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This CHK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.

Isotyp

Ig Fraction
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibody
CHEK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
CHEK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibody
CHEK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibody
CHEK1 Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody
CHEK1 Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Rind (Kuh), Pferd, Meerschweinchen WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 Reaktivität: Maus, Huhn ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 Reaktivität: Human WB, ELISA, IHC, IF Wirt: Kaninchen Monoclonal 2F2 unconjugated Recombinant Antibody

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 Reaktivität: Human, Maus WB, ELISA Wirt: Maus Monoclonal 2G1D5 unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
CHEK1 Reaktivität: Human, Maus, Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB: 1:1000. IHC-P: 1:50~100. DB: 1:500

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Awate, De Benedetti: "TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint." in: BMC molecular biology, Vol. 17, pp. 3, (2016) (PubMed).
Target

CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung

CHK1

Hintergrund

Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.

Molekulargewicht

54434

Gen-ID

1111

NCBI Accession

NP_001107593, NP_001107594, NP_001231775, NP_001265

UniProt

O14757

Pathways

p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur