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PCNA Antikörper (AA 89-117)

Der Kaninchen Polyklonal anti-PCNA Antikörper wird verwendet zum Nachweis von PCNA in Proben von Human. Er wurde validiert für WB, FACS, IF und IHC (p).

Western blot analysis of PCNA Antibody (Center) (ABIN389345 and ABIN2839454) in Jurkat(lane 1), Hela(lane 2), 293(lane 3) cell line lysates (35 μg/lane). (PCNA Antikörper (AA 89-117))

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (Center) (ABIN389345 and ABIN2839454) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PCNA Antikörper (AA 89-117))

IHC (p)

Confocal immunofluorescent analysis of PCNA Antibody (Center) (ABIN389345 and ABIN2839454) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PCNA Antikörper (AA 89-117))

PCNA Antibody (Center) (ABIN389345 and ABIN2839454) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (PCNA Antikörper (AA 89-117))

FACS

4 Abbildungen

Produktnummer ABIN389345

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Kurzübersicht für PCNA Antikörper (AA 89-117) (ABIN389345)

Target

Alle PCNA Antikörper anzeigen

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität

Human

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser PCNA Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

RB17485

Discover our top product PCNA Primärantikörper

Bindungsspezifität
16

16

16

16

15

12

8

6

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 89-117

Homologie

B, C, Ha, Pr, M, Rat

Aufreinigung

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PCNA.

Isotyp

Ig Fraction
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 151-261) antibody
PCNA Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA Reaktivität: Human WB, IHC, IF, IP Wirt: Kaninchen Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 231-261), (C-Term) antibody
PCNA Reaktivität: Human WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB17486 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA Reaktivität: Human WB, IHC, IF, IP, IHC (p), ICC, IHC (fro), FISH Wirt: Kaninchen Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA Reaktivität: Human WB, ELISA, IF, IP Wirt: Maus Monoclonal 1G7 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal S1 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibody
PCNA Reaktivität: Human, Maus, Ratte WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 7H4F8 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibody
PCNA Reaktivität: Human, Affe WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 7H4F8 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 201-261) antibody
PCNA Reaktivität: Human, Maus, Ratte, Huhn WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibody
PCNA Reaktivität: Human, Maus, Ratte, Schwein, Hund, Kaninchen, Rhesusaffen WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Target

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung

PCNA

Hintergrund

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

Molekulargewicht

28769

Gen-ID

5111

NCBI Accession

NP_002583, NP_872590

UniProt

P12004

Pathways

Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie