ATG5 Antikörper (C-Term)

Produktnummer ABIN388519

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ATG5 in WB, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 29+ Publikationen erwähnt.

Kurzübersicht für ATG5 Antikörper (C-Term) (ABIN388519)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB7547

Produktdetails anti-ATG5 Antikörper

Bindungsspezifität: AA 209-238, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the C-terminal region of human ATG5.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF: 1:25. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-ATG5 Antikörper (ABIN388519)

Zhang, Michel, Lenz, Friedel, Köster, dHedouville, Tönges, Urlaub, Bähr, Lingor, Koch: "Calpain-mediated cleavage of collapsin response mediator protein-2 drives acute axonal degeneration." in: Scientific reports, Vol. 6, pp. 37050, (2018) (PubMed).

Radogna, Cerella, Gaigneaux, Christov, Dicato, Diederich: "Cell type-dependent ROS and mitophagy response leads to apoptosis or necroptosis in neuroblastoma." in: Oncogene, Vol. 35, Issue 29, pp. 3839-53, (2017) (PubMed).

Kessel, Evans: "Promotion of Proapoptotic Signals by Lysosomal Photodamage: Mechanistic Aspects and Influence of Autophagy." in: Photochemistry and photobiology, Vol. 92, Issue 4, pp. 620-3, (2017) (PubMed).

Zhang, Gao, Zhang, Song, Cheng, Zhou: "The germline-enriched Ppp1r36 promotes autophagy." in: Scientific reports, Vol. 6, pp. 24609, (2017) (PubMed).

Mathai, Meijer, Simonsen: "Studying Autophagy in Zebrafish." in: Cells, Vol. 6, Issue 3, (2017) (PubMed).

Du, Ren, Yao, Duan, Zhao, Du, Xiao, Duan, Shi: "Sphingosine kinase 1 protects renal tubular epithelial cells from renal fibrosis via induction of autophagy." in: The international journal of biochemistry & cell biology, Vol. 90, pp. 17-28, (2017) (PubMed).

Paquet, Nicoll, Love, Mouton-Liger, Holmes, Hugon, Boche: "Downregulated apoptosis and autophagy after anti-Aβ immunotherapy in Alzheimer's disease." in: Brain pathology (Zurich, Switzerland), (2017) (PubMed).

Wang, Li, Wang, Ma, Chuai, Cao, Yang: "High glucose environment inhibits cranial neural crest survival by activating excessive autophagy in the chick embryo." in: Scientific reports, Vol. 5, pp. 18321, (2016) (PubMed).

Varga, Fodor, Vellai: "Autophagy in zebrafish." in: Methods (San Diego, Calif.), Vol. 75, pp. 172-80, (2015) (PubMed).

Ribas, Schnepf, Challagundla, Koch, Bähr, Lingor: "Early and sustained activation of autophagy in degenerating axons after spinal cord injury." in: Brain pathology (Zurich, Switzerland), Vol. 25, Issue 2, pp. 157-70, (2015) (PubMed).

Hsin, Ou, Wu, Jan, Hsiao, Lin, Ko: "GMI, an Immunomodulatory Protein from Ganoderma microsporum, Potentiates Cisplatin-Induced Apoptosis via Autophagy in Lung Cancer Cells." in: Molecular pharmaceutics, Vol. 12, Issue 5, pp. 1534-43, (2015) (PubMed).

Xin, Xiao, Ma, He, Yao, Liu et al.: "Coxsackievirus B3 induces crosstalk between autophagy and apoptosis to benefit its release after replicating in autophagosomes through a mechanism involving caspase cleavage of autophagy-related ..." in: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, Vol. 26, pp. 95-102, (2014) (PubMed).

Degtyarev, Reichelt, Lin: "Novel quantitative autophagy analysis by organelle flow cytometry after cell sonication." in: PLoS ONE, Vol. 9, Issue 1, pp. e87707, (2014) (PubMed).

Yao, Jia, Shelby, Ganios, Feathers, Thompson, Zacks: "Circadian and noncircadian modulation of autophagy in photoreceptors and retinal pigment epithelium." in: Investigative ophthalmology & visual science, Vol. 55, Issue 5, pp. 3237-46, (2014) (PubMed).

Al-Zeer, Al-Younes, Lauster, Abu Lubad, Meyer: "Autophagy restricts Chlamydia trachomatis growth in human macrophages via IFNG-inducible guanylate binding proteins." in: Autophagy, Vol. 9, Issue 1, pp. 50-62, (2013) (PubMed).

Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, (2012) (PubMed).

Chiu, Chen, Ho, Wang: "Arsenic trioxide enhances the radiation sensitivity of androgen-dependent and -independent human prostate cancer cells." in: PLoS ONE, Vol. 7, Issue 2, pp. e31579, (2012) (PubMed).

Hu, Zhang, Zhang: "Expression pattern and functions of autophagy-related gene atg5 in zebrafish organogenesis." in: Autophagy, Vol. 7, Issue 12, pp. 1514-27, (2012) (PubMed).

Norberg, Karlsson, Korenovska, Szydlowski, Silberberg, Uhlén, Orrenius, Zhivotovsky: "Critical role for hyperpolarization-activated cyclic nucleotide-gated channel 2 in the AIF-mediated apoptosis." in: The EMBO journal, Vol. 29, Issue 22, pp. 3869-78, (2010) (PubMed).

Dou, Chattopadhyay, Pan, Guerriero, Jiang, Ballou, Yue, Lin, Zong: "The class IA phosphatidylinositol 3-kinase p110-beta subunit is a positive regulator of autophagy." in: The Journal of cell biology, Vol. 191, Issue 4, pp. 827-43, (2010) (PubMed).

Details zu ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: ATG5

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Molekulargewicht: 32447

Gen-ID: 9474

NCBI Accession: NP_004840

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie