CD25 Antikörper (Biotin)

Produktnummer ABIN375432

Dieses Anti-CD25-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von CD25 in FACS. Geeignet für Maus.

Kurzübersicht für CD25 Antikörper (Biotin) (ABIN375432)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD25 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: 3C7

Produktdetails anti-CD25 Antikörper (Biotin)

Spezifität: Mouse CD25, Mr 55 kDa

Produktmerkmale: Rat Anti-Mouse CD25-BIOT

Aufreinigung: Purified

Immunogen: IL-2 dependent BALB/c helper T cell clone HT2

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Neut - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 3 g/106 cells PE, APC, SPRD, PE/CY7, and AF700 conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Kommentare:

Inhibition of IL-2 or mitogen-induced T cell proliferation, In vitro blocking of IL-2 binding

Probenmenge: 1 mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25

Hintergrund: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. It is expressed at a higher level on CD4 + CD8 + thymocytes. It is also expressed on cultured epidermal Langerhans cells. The biochemical features of murine CD25 have been characterized in detail.

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation