NOD2 Antikörper (Internal Region)

Produktnummer ABIN374736

Der Ziege Polyklonal anti-NOD2 Antikörper (ABIN374736) detektiert spezifisch NOD2 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für NOD2 Antikörper (Internal Region) (ABIN374736)

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NOD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NOD2 Antikörper

Bindungsspezifität: Internal Region

Sequenz: KFRFTDRERH CSPTD

Spezifität: This antibody recognizes CARD15 / NOD2.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence Genename: CARD15

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NOD2

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Andere Bezeichnung: CARD15 / NOD2

Hintergrund: CARD15 (Nod2) is a member of the Nod1/Apaf-1 family of intracellular proteins that contain an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and a C-terminal regulatory domain. CARD15 induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It also confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Unlike Nod1, this homologue is primary expressed in monocytes. Defects in CARD15 are associated with Blau Syndrome (BS) and Crohn's Disease (CD), and are a cause of susceptibility to ulcerative colitis.Synonyms: Caspase recruitment domain-containing protein 15, IBD1, Inflammatory bowel disease protein 1, Nucleotide-binding oligomerization domain-containing protein 2

Gen-ID: 64127, 9606

UniProt: Q9HC29

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome