Complement Factor H Antikörper (Internal Region)

Produktnummer ABIN374588

Der Ziege Polyklonal Anti-Complement Factor H-Antikörper wurde für WB validiert. Er ist geeignet, Complement Factor H in Proben von Human zu detektieren.

Kurzübersicht für Complement Factor H Antikörper (Internal Region) (ABIN374588)

Target: Complement Factor H (CFH)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Complement Factor H Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Complement Factor H Antikörper

Bindungsspezifität: Internal Region

Spezifität: This antibody is expected to recognize isoform a of Complement factor H (CFH) only.

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/32000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Complement Factor H

Target: Complement Factor H (CFH)

Hintergrund: The complement Factor H protein is secreted into the bloodstream and acts in the regulation of complement activation. Mutations leading to changes in this protein have been linked with HUS (hemolytic-uremic syndrome) and chronic hypocomplementemic nephropathy. Factor H is mainly synthesised in the liver but also in macrophages and endothelium. It is primarily aplasma glycoprotein but is also found in platelets and there is a membrane bound form on some leukocytes. Consisting of a single polypeptide, the major form of Factor H has a molecular weight of 155 kDa. There are two truncated forms, a non-glycosylated 49 kDa form and a glycosylated 39-43 kDaform. Plasma concentrations are in the range 200-600 mg/L for the 155 kDa form and 1-5 mg/L for thetruncated forms. Factor H is a major regulatory protein of the complement system. By binding to C3b it either displacesor prevents the binding of Bb (activated Factor B). When bound to Factor H, C3b is susceptible tocleavage by Factor 1 to yield iC3b. Factor H is released or modified following this cleavage. The regulatory role of Factor H is essential because C3bBb is not only a C5 convertase but a C3 convertaseand so has a positive feedback effect, potentially consuming the entire C3 pool if unregulated.Synonyms: CFH, H factor 1, HF, HF1, HF2

Gen-ID: 3075

NCBI Accession: NP_000177

UniProt: P08603

Pathways: Komplementsystem, Cellular Response to Molecule of Bacterial Origin