KPNA1 Antikörper (N-Term)

Produktnummer ABIN374391

Produktdetails anti-KPNA1 Antikörper

Target: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KPNA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: TTPGKENFRL KSY-C

Spezifität: This antibody detects Importin subunit alpha-1 at N-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Dog Pig, Cow.
Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_002255.3 Genename: KPNA1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 60 kDa band observed in lysates of cell lines HEK293, HeLaand HEPG2 (calculated MW of 60.0 kDa according to NP_002255.1).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu KPNA1

Target: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Andere Bezeichnung: KPNA1 / Importin alpha-1 (KPNA1 Produkte)

Hintergrund: Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. SRP1 (nucleoprotein interactor 1, NPI 1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein's nuclear localization signal (Moroianu J. Crit 1997). Both subunits of karyopherin are required for protein docking at the NPC, the alpha subunit binds the substrate and the beta subunit acts as an adaptor (Moroianu J, et al. 1995). Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin ß and promotes the release of both subunits from the docking site (Moroianu J. Crit 1997). The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC (Moroianu J. Crit 1997)Synonyms: IPOA5, Karyopherin alpha 1, NPI-1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1-beta

Gen-ID: 3836

NCBI Accession: NP_002255

UniProt: P52294

Pathways: M Phase, Protein targeting to Nucleus