Src Antikörper (AA 527-531)

Produktnummer ABIN362786

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Src in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für Src Antikörper (AA 527-531) (ABIN362786)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Src Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Src Antikörper

Bindungsspezifität: AA 527-531

Spezifität: The antibody detects endogenous level of total Src protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 527-531 (P-Q-Y-Q-P) derived from Human Src. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-Src Antikörper (ABIN362786)

Zheng, Xia, Hawke, Halle, Tremblay, Gao, Zhou, Aldape, Cobb, Xie, He, Lu: "FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST." in: Molecular cell, Vol. 35, Issue 1, pp. 11-25, (2009) (PubMed).

Details zu Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Andere Bezeichnung: Src

Hintergrund: Proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme, phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Molekulargewicht: 60 kDa

NCBI Accession: NP_005408

UniProt: P12931

Pathways: JAK-STAT Signalweg, Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling