Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Spezifität
The antibody detects endogenous level of PKR only when phosphorylated at threonine 446.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pThr446 (K-R-T (p) -R-S) derived from Human PKR. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.