Connexin 43/GJA1 Antikörper (pSer368)

Produktnummer ABIN362324

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Connexin 43/GJA1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für Connexin 43/GJA1 Antikörper (pSer368) (ABIN362324)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Connexin 43/GJA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Connexin 43/GJA1 Antikörper

Bindungsspezifität: pSer368

Spezifität: The antibody detects endogenous level of Connexin43 only when phosphorylated at serine 368.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer368 (R-A-S (p) -S-R) derived from Human Connexin 43. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100  

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Andere Bezeichnung: Connexin 43

Hintergrund: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Molekulargewicht: 43 kDa

NCBI Accession: NP_000156

UniProt: P17302

Pathways: MAPK Signalweg, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization