CDK1 Antikörper (pTyr15)

Produktnummer ABIN362298

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-CDK1 Antikörper wird verwendet zum Nachweis von CDK1 in Proben von Human, Maus und Ratte.

Kurzübersicht für CDK1 Antikörper (pTyr15) (ABIN362298)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK1 Antikörper ist unkonjugiert

Applikation: Bitte anfragen

Produktdetails anti-CDK1 Antikörper

Bindungsspezifität: pTyr15

Spezifität: The antibody detects endogenous level of CDC2 only when phosphorylated at tyrosine 15.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr15 (G-T-Y (p) -G-V) derived from Human CDC2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-CDK1 Antikörper (ABIN362298)

Kubara, Kernéis-Golsteyn, Studény, Lanser, Meijer, Golsteyn: "Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents." in: The Biochemical journal, Vol. 446, Issue 3, pp. 373-81, (2012) (PubMed).

Yao, Li, Liu, Huang, Guo: "SUMOylation-regulated protein phosphorylation, evidence from quantitative phosphoproteomics analyses." in: The Journal of biological chemistry, Vol. 286, Issue 31, pp. 27342-9, (2011) (PubMed).

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDC2

Hintergrund: Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Molekulargewicht: 34 kDa

NCBI Accession: NP_001163

UniProt: P06493

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA