JAK1 Antikörper (pTyr1022)

Produktnummer ABIN362146

Zitiert in 2+ Publikationen. Der Kaninchen Polyklonal anti-JAK1 Antikörper (ABIN362146) detektiert spezifisch JAK1 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für JAK1 Antikörper (pTyr1022) (ABIN362146)

Target: JAK1 (Janus Kinase 1 (JAK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-JAK1 Antikörper

Bindungsspezifität: pTyr1022

Spezifität: The antibody detects endogenous level of JAK1 only when phosphorylated at tyrosine 1022.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr1022 (K-E-Y (p) -Y-T) derived from Human JAK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-JAK1 Antikörper (ABIN362146)

Liu, Huang, Zeng, Chen, Huang, Guo, Liu, Xu, Mo, Li: "Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway." in: Journal of cancer research and clinical oncology, Vol. 137, Issue 11, pp. 1629-40, (2011) (PubMed).

Cha, Moon, Lee, Ahn, Lee, Lee, Fornace, Kim, Cha, Park: "Zap70 functions to maintain stemness of mouse embryonic stem cells by negatively regulating Jak1/Stat3/c-Myc signaling." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 9, pp. 1476-86, (2010) (PubMed).

Details zu JAK1

Target: JAK1 (Janus Kinase 1 (JAK1))

Andere Bezeichnung: JAK1

Hintergrund: Tyrosine kinase of the non-receptor type, involved in the IFN-a/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Molekulargewicht: 130 kDa

NCBI Accession: NP_002218

UniProt: P23458

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Hepatitis C, Toll-Like Receptors Cascades, Unfolded Protein Response