MEF2A Antikörper (pThr312)

Produktnummer ABIN361950

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-MEF2A Antikörper wird verwendet zum Nachweis von MEF2A in Proben von Human, Maus und Ratte. Er wurde validiert für IHC und IF.

Kurzübersicht für MEF2A Antikörper (pThr312) (ABIN361950)

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MEF2A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-MEF2A Antikörper

Bindungsspezifität: pThr312

Spezifität: The antibody detects endogenous level of MEF2A only when phosphorylated at threonine 312.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr312 (L-A-T (p) -P-V) derived from Human MEF2A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-MEF2A Antikörper (ABIN361950)

Satoh, Ohnishi, Sato, Takeyama, Iemura, Natsume, Shibuya: "Nemo-like kinase-myocyte enhancer factor 2A signaling regulates anterior formation in Xenopus development." in: Molecular and cellular biology, Vol. 27, Issue 21, pp. 7623-30, (2007) (PubMed).

Details zu MEF2A

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Andere Bezeichnung: MEF2A

Hintergrund: The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain

Molekulargewicht: 54 kDa

NCBI Accession: NP_001124

UniProt: Q02078

Pathways: Neurotrophin Signalübertragung, Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades