Tryptophan Hydroxylase 1 Antikörper (pSer260)

Produktnummer ABIN361499

Der Kaninchen Polyklonal Anti-Tryptophan Hydroxylase 1-Antikörper wurde für WB validiert. Er ist geeignet, Tryptophan Hydroxylase 1 in Proben von Ratte zu detektieren.

Kurzübersicht für Tryptophan Hydroxylase 1 Antikörper (pSer260) (ABIN361499)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Tryptophan Hydroxylase 1 Antikörper

Bindungsspezifität: pSer260

Spezifität: Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser260.

Kreuzreaktivität: Human, Ratte (Rattus)

Homologie: bovine, canine, chicken, mouse, zebra fish

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Andere Bezeichnung: TPH1

Hintergrund: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000). Anti-Phospho Ser260 Tryptophan Hydroxylase Western blot of rat brainstem lysate showing specific immunolabeling of the ~55k TPH protein phosphorylated at Ser260. The labeling is specifically blocked by the phosphopeptide (Phos-pep) used as antigen. The corresponding non-phosphopeptide (Nonphos-pep) did not block the immunolabeling.

Molekulargewicht: '55 kDa

Gen-ID: 24848

UniProt: P09810

Pathways: Feeding Behaviour