ERK1 Antikörper (N-Term)

Produktnummer ABIN359630

Der Kaninchen Polyklonal anti-ERK1 Antikörper wird verwendet zum Nachweis von ERK1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für ERK1 Antikörper (N-Term) (ABIN359630)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-ERK1 Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody reacts to ERK1.

Aufreinigung: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ERK1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Andere Bezeichnung: MAPK3 / ERK1

Hintergrund: ERK1, a member of the MAP kinase subfamily of Ser/Thr protein kinases, is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. It phosphorylates EIF4EBP1 and is required for initiation of translation. The protein also phosphorylates microtubule-associated protein-2 (MAP2). ERK1 is activated and tyrosine phosphorylated in response to insulin and NGF.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK

Gen-ID: 5595, 9606

UniProt: P27361

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteine