SPHK2 Antikörper (N-Term)

Produktnummer ABIN359440

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SPHK2 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SPHK2 Antikörper (N-Term) (ABIN359440)

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPHK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-SPHK2 Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SPHK2.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Andere Bezeichnung: Sphingosine Kinase 2 / SPK2

Hintergrund: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.Synonyms: SK2, SPHK2

Molekulargewicht: 69217 Da

Gen-ID: 56848, 9606

UniProt: Q9NRA0

Pathways: VEGF Signaling