c-MET Antikörper (pTyr1349)

Produktnummer ABIN358164

Der Kaninchen Polyklonal anti-c-MET Antikörper (ABIN358164) detektiert spezifisch c-MET in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für c-MET Antikörper (pTyr1349) (ABIN358164)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser c-MET Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-c-MET Antikörper

Bindungsspezifität: pTyr1349

Spezifität: This antibody detects HGF Receptor (MET) pTyr1349. Predicted to cross react with Mouse and Rabbit (100 % Antigen Homology).

Aufreinigung: Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a 4=4 Chain MAPS conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1349 of human MET. Synthetic phosphopeptide was selected from region 1345~1357 of human MET.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Andere Bezeichnung: HGF Receptor

Hintergrund: The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.Synonyms: HGF/SF receptor, Hepatocyte growth factor receptor, MET, Met proto-oncogene tyrosine kinase, Scatter factor receptor, c-Met

Molekulargewicht: 155527 Da

Gen-ID: 4233, 9606

UniProt: P08581

Pathways: RTK Signalweg, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor