CHEK1 Antikörper

Produktnummer ABIN356395

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CHEK1 in WB und EIA. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für CHEK1 Antikörper (ABIN356395)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 2G1D5

Produktdetails anti-CHEK1 Antikörper

Spezifität: This antibody is specific to ChK1.

Produktmerkmale: Synonyms: CHEK1, CHEK-1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog

Aufreinigung: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This monoclonal antibody is generated from mice immunized with Ni-NTA purifiedrecombinant protein CHK1 expressed in E. Coli strain M15.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10,000. Western blotting: 1/1,000-1/4,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: CHK1

Hintergrund: Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The protein exhibits altered mobility in immunoblotting when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Gen-ID: 1111

UniProt: O14757

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur