Axin Antikörper (AA 850-862)

Produktnummer ABIN351463

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Axin in ELISA, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Affe, Rind (Kuh), Fledermaus, Hamster, Pferd und Zebrafisch (Danio rerio).

Kurzübersicht für Axin Antikörper (AA 850-862) (ABIN351463)

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh), Fledermaus, Hamster, Pferd, Zebrafisch (Danio rerio)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Axin Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Axin Antikörper

Bindungsspezifität: AA 850-862

Spezifität: Human AXIN1. This antibody is expected to recognise both reported isoforms.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Horse, Opossum, Stickleback, Pufferfish, Zebrafish (100%) Turkey, Chicken (92%) Xenopus, Sea anemone (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-FEEKIIGKVEKVD from the C-terminus of human AXIN1 (NP_003493.1, NP_851393.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Horse, Opossum, Stickleback, Pufferfish, Zebrafish (100%), Turkey, Chicken (92%), Xenopus, Sea anemone (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Andere Bezeichnung: AXIN1 / Axin-1

Hintergrund: Name/Gene ID: AXIN1

Synonyms: AXIN1, Axis inhibitor 1, AXIN, Axin 1, Axis inhibition protein 1, Fused, mouse, homolog of, HAxin, PPP1R49, Axin-1

Gen-ID: 8312

NCBI Accession: NP_003493, NP_851393

UniProt: O15169

Pathways: WNT Signalweg, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling