SQSTM1 Antikörper (AA 300-360)

Produktnummer ABIN350828

Der Kaninchen Polyklonal Anti-SQSTM1-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, SQSTM1 in Proben von Human zu detektieren.

Kurzübersicht für SQSTM1 Antikörper (AA 300-360) (ABIN350828)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SQSTM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SQSTM1 Antikörper

Bindungsspezifität: AA 300-360

Verwendungszweck: Rabbit antibody to SQSTM1 (300-360)

Spezifität: Specific for sequestosome-1.

Kreuzreaktivität: Human

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide from AA 300-360 of human SQSTM1 has been used as the antigen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC (both on frozen,paraffin embedded tissues) WB. Use at a concentration of 10-50 μg,ml. The optimal concentration should be determined by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Andere Bezeichnung: SQSTM1

Hintergrund: FUNCTION: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation apoptosis immune response and regulation of K(+) channels. SUBCELLULAR LOCATION: Cytoplasm. Late endosome. Nucleus. Note=Sarcomere. In cardiac muscles localizes to the sarcomeric band. Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells accumulates in Mallory bodies associated with alcoholic hepatitis Wilson disease indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. TISSUE SPECIFICITY: Ubiquitously expressed.

UniProt: Q13501

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Autophagie