TRIM29 Antikörper (acLys116)

Produktnummer ABIN349613

Hersteller Produkt- Nr.: 600-401-b16

Dieses Anti-TRIM29-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TRIM29 in WB und ELISA. Geeignet für Human, Rind (Kuh), Pferd, Makake und Schimpanse. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für TRIM29 Antikörper (acLys116) (ABIN349613)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Reaktivität: Human, Rind (Kuh), Pferd, Makake, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRIM29 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TRIM29 Antikörper

Bindungsspezifität: acLys116

Hersteller Produkt- Nr.: 600-401-b16

Hersteller: Rockland

Verwendungszweck: ATDC Ac-K116 Antibody

Kreuzreaktivität (Details): This antibody reacts with over-expressed, acetylated lysine ATDC protein.

Produktmerkmale: Synonyms: rabbit anti-ATDC Ac-K116 Antibody, rabbit anti-ATDC acetylated Lys116 Antibody, Ataxia-telangiectasia group D associated protein antibody, FLJ36085 antibody, TRIM 29 antibody, Tripartite motif containing protein 29 antibody, TRIM-29

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.   Specific conditions for reactivity and detection of ATDC Ac-K116 should be optimized by the end user.  Expect a band approximately ~66 kDa in size corresponding to ATDC Ac-K116 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 to 3,000

ELISA Dilution: 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.67 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-TRIM29 Antikörper (ABIN349613)

Yuan, Peng, Radhakrishnan, Seto: "Histone deacetylase 9 (HDAC9) regulates the functions of the ATDC (TRIM29) protein." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 39329-38, (2011) (PubMed).

Yuan, Villagra, Peng, Coppola, Glozak, Sotomayor, Chen, Lane, Seto: "The ATDC (TRIM29) protein binds p53 and antagonizes p53-mediated functions." in: Molecular and cellular biology, Vol. 30, Issue 12, pp. 3004-15, (2010) (PubMed).

Details zu TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Andere Bezeichnung: TRIM29

Hintergrund: Background: Ataxia-telangiectasia group D-associated protein (ATDC), also called tripartite motif-containing protein 29 (TRIM29), is a novel Histone deacetylase (HDAC) associated protein. Its function is tightly regulated by HDAC. ATDC Lysine 116 (K116) is acetylated and has a significant functional role in regulating cell survival and tumorigenesis. ATDC is expressed in placenta, prostate and thymus, and is over expressed in pancreatic and cervical tumors. Its function in tumor cells is not fully understood. It is constitutively phosphorylated by PKC on serine/threonine in A431 cells. The ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or heterodimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation.

Gen-ID: 23650, 17402909

UniProt: Q14134

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus