C5AR1 Antikörper

Produktnummer ABIN337372

Dieses Maus Monoklonal-Antikörper erkennt spezifisch C5AR1 in FACS, IHC, IHC (p), Func und Neut. Er zeigt eine Reaktivität gegenüber Human und Kaninchen.

Kurzübersicht für C5AR1 Antikörper (ABIN337372)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reaktivität: Human, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C5AR1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Neutralization (Neut)

Produktdetails anti-C5AR1 Antikörper

Marke: IHC-plus™

Spezifität: Recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone 3H1738 was raised against a synthetic peptide Met1 -Asn31 and has recently been shown to recognize the heptameric peptide (D15DKDTLD21). Clone 3H1738 inhibits C5a binding to its receptor. Species cross-reactivity: Rabbit.

Aufreinigung: Ion exchange chromatography

Immunogen: C5aR - peptide: Met1 - Asn31.

Type of Immunogen: Synthetic peptide

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:50), Func, IHC, IHC-P (10 μg/mL), Neut

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: 1:50. Use 20 μL to label 5x10^5 cells in 100 μL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 40 % glycerol. No preservative added.

Konservierungsmittel: Azide free

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Andere Bezeichnung: C5AR1 / CD88 / C5a Receptor

Hintergrund: Name/Gene ID: C5AR1
Subfamily: Anaphylatoxin
Family: GPCR

Synonyms: C5AR1, C5A, C5a-R, C5a ligand, C5a receptor, CD88, C5a anaphylatoxin receptor, C5AR, C5R1, CD88 antigen

Gen-ID: 728

UniProt: P21730

Pathways: Komplementsystem