Complement Factor H Antikörper (AA 577-588)

Produktnummer ABIN337307

Der Ziege Polyklonal anti-Complement Factor H Antikörper wird verwendet zum Nachweis von Complement Factor H in Proben von Human. Er wurde validiert für WB, IHC, ELISA und IHC (p).

Kurzübersicht für Complement Factor H Antikörper (AA 577-588) (ABIN337307)

Target: Complement Factor H (CFH)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Complement Factor H Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Complement Factor H Antikörper

Bindungsspezifität: AA 577-588

Spezifität: Human CFH / Factor H. This antibody is expected to recognize isoform a (NP_000177.2) only.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-HLVPDRKKDQYK from an internal region of human CFH / Factor H (NP_000177.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Complement Factor H

Target: Complement Factor H (CFH)

Andere Bezeichnung: CFH / Complement Factor H

Hintergrund: Name/Gene ID: CFH

Synonyms: CFH, Adrenomedullin binding protein, AHUS1, AMBP1, ARMS1, ARMD4, Beta-1-H-globulin, Factor H, Factor H-like 1, HF, HF2, H factor 1, H factor 2 (complement), HUS, HF1, Beta-1H, CFHL3, Complement factor H, H factor 1 (complement)

Gen-ID: 3075

NCBI Accession: NP_000177

UniProt: P08603

Pathways: Komplementsystem, Cellular Response to Molecule of Bacterial Origin