PRAS40 Antikörper

Produktnummer ABIN337263

Dieses Anti-PRAS40-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PRAS40 in WB, IHC, IHC (p) und IP. Geeignet für Human.

Kurzübersicht für PRAS40 Antikörper (ABIN337263)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PRAS40 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-PRAS40 Antikörper

Marke: IHC-plus™

Spezifität: Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.

Aufreinigung: Protein A/G purified

Immunogen: Recombinant full length human PRAS40 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), IP, WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 μg/mL. Positive control: HeLa cells, Jurkat cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide, 1 % BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Andere Bezeichnung: AKT1S1 / PRAS40

Hintergrund: Name/Gene ID: AKT1S1

Synonyms: AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, Lobe

Gen-ID: 84335

UniProt: Q96B36

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Cell Size, Autophagie, BCR Signaling, Warburg Effekt