CDK2 Antikörper

Produktnummer ABIN337208

Der Maus Monoklonal Anti-CDK2 Antikörper wurde für WB, IHC, ELISA, IP, IF und IHC (p) validiert. Er ist geeignet, CDK2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für CDK2 Antikörper (ABIN337208)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CDK2 Antikörper

Spezifität: Recognizes human Cdk2. Species cross-reactivity: mouse and rat.

Aufreinigung: Protein A purified

Immunogen: Human recombinant cdk2 protein.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a, IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IF, IHC, IHC-P (5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: 2 μg/mg protein lysate. (Denatured only) Use Protein A. Western Blot: 1-2 μg/mL for 2hrs at RT. Positive control: HeLa cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Andere Bezeichnung: CDK2

Hintergrund: Name/Gene ID: CDK2
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK2, Cell division protein kinase 2, CDC2-related protein kinase, p33 protein kinase, p33(CDK2), CDKN2, Cyclin-dependent kinase 2

Gen-ID: 1017

UniProt: P24941

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA