XBP1 Antikörper (AA 358-372)

Produktnummer ABIN337110

Der Ziege Polyklonal anti-XBP1 Antikörper (ABIN337110) detektiert spezifisch XBP1 in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Affe.

Kurzübersicht für XBP1 Antikörper (AA 358-372) (ABIN337110)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser XBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-XBP1 Antikörper

Bindungsspezifität: AA 358-372

Spezifität: Human XBP1. This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Panda (93%) Pig, Opossum (87%) Bat, Turkey, Pufferfish (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-NHSWEDTFANELFPQ from an internal region of human XBP1 (NP_001073007.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Panda (93%), Pig, Opossum (87%), Bat, Turkey, Pufferfish (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:4000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Andere Bezeichnung: XBP1

Hintergrund: Name/Gene ID: XBP1

Synonyms: XBP1, X-box binding protein 1, X-box-binding protein 1, XBP2, TREB5, XBP-1

Gen-ID: 7494

NCBI Accession: NP_001073007

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response