XBP1 Antikörper (N-Term)

Produktnummer ABIN3043963

Produktdetails anti-XBP1 Antikörper

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Bindungsspezifität: AA 59-85, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser XBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for X-box-binding protein 1(XBP1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: RKRQRLTHLS PEEKALRRKL KNRVAAQ

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for X-box-binding protein 1(XBP1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: X-box binding protein 1
Protein Name: X-box-binding protein 1

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human XBP1 (59-85 aa RKRQRLTHLSPEEKALRRKLKNRVAAQ), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for XBP1 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Andere Bezeichnung: XBP1 (XBP1 Produkte)

Synonyme: treb5 antikoerper, xXBP1 antikoerper, xbp2 antikoerper, XBP1P1 antikoerper, XBPP1 antikoerper, xbp1 antikoerper, TREB5 antikoerper, XBP-1 antikoerper, XBP2 antikoerper, D11Ertd39e antikoerper, TREB-5 antikoerper, HTF antikoerper, cb918 antikoerper, id:ibd1195 antikoerper, trebf antikoerper, wu:fb02h05 antikoerper, wu:fb63f10 antikoerper, xbp1a antikoerper, xbp1b antikoerper, X-box binding protein 1 antikoerper, X-box binding protein 1 L homeolog antikoerper, xbp1 antikoerper, XBP1 antikoerper, Xbp1 antikoerper, xbp1.L antikoerper

Hintergrund: XBP1, also known as X-box binding protein 1, is a protein which in humans is encoded by the XBP1 gene. It encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

Synonyms: Tax responsive element binding protein 5 antibody|Tax-responsive element-binding protein 5 antibody|TREB5 antibody|X box binding protein 1 antibody|X box binding protein 2 antibody| X-box-binding protein 1 antibody|XBP 1 antibody|XBP-1 antibody|XBP1 antibody|XBP1_HUMAN antibody|XBP2 antibody|XBP2 antibody

Gen-ID: 7494

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response