Dynactin 1 Antikörper (AA 1266-1278)

Produktnummer ABIN337005

Der Ziege Polyklonal anti-Dynactin 1 Antikörper (ABIN337005) detektiert spezifisch Dynactin 1 in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Pferd, Affe und Hamster.

Kurzübersicht für Dynactin 1 Antikörper (AA 1266-1278) (ABIN337005)

Target: Dynactin 1 (DCTN1)

Reaktivität: Human, Maus, Pferd, Affe, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Dynactin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Dynactin 1 Antikörper

Bindungsspezifität: AA 1266-1278

Spezifität: Human DCTN1. This antibody is expected to recognise all reported isoforms (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Horse (100%) Rat, Bovine, Dog, Panda, Rabbit, Pig (92%) Bat (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QEQLHQLHSRLIS from the C-terminus of human DCTN1 (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Horse (100%), Rat, Bovine, Dog, Panda, Rabbit, Pig (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (1:128000, 0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Dynactin 1

Target: Dynactin 1 (DCTN1)

Andere Bezeichnung: DCTN1 / Dynactin 1

Hintergrund: Name/Gene ID: DCTN1

Synonyms: DCTN1, DAP-150, Dynactin subunit 1, DP-150, Dynactin 1, HMN7B, p150-glued, p135

Gen-ID: 1639

NCBI Accession: NP_004073, NP_075408, NP_001128512, NP_001128513

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling