BRCA2 Antikörper (AA 2587-2601)

Produktnummer ABIN318412

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BRCA2 in IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Affe, Cat, Hund und Pferd.

Kurzübersicht für BRCA2 Antikörper (AA 2587-2601) (ABIN318412)

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reaktivität: Human, Ratte, Maus, Affe, Cat, Hund, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRCA2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-BRCA2 Antikörper

Bindungsspezifität: AA 2587-2601

Sequenz: LIPSNDGKAG KEEFY

Spezifität: This antibody is specific for Breast Cancer Type 2 susceptibility protein (BRCA2), a tumour-suppressor protein reported to be involved in double-strand break repair, homologous recombination and S-phase checkpoint activation. Based on sequence similarity, is expected to react with: Mouse, Rat, Horse, Cat, Chimpanzee, Rhesus Monkey and Dog.

Aufreinigung: Purified IgG prepared by affinity chromatography on Protein G

Immunogen: Synthetic peptide corresponding to amino acids 2587-2601 of the human BRCA2 precursor, conjugated to Keyhole Limpet Haemocyanin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/25-1/100): This product requires antigenretrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 5.0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Andere Bezeichnung: BRCA2 / FANCD1

Hintergrund: BRCA 2 is localized to chromosome 13q12 - q13. Loss of heterozygosity of 13q is observed in 25 % of sporadic breast tumors, which indicates that BRCA 2 might be a tumor suppressor gene. BRCA 2 confers only a low ovarian cancer risk. Reportedly, in MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA 2 RNA lowers in G0 and early G1 phases then gets up-regulated at the G1 / S phase junction.Synonyms: Breast cancer type 2 susceptibility protein, FACD, Fanconi anemia group D1 protein

Gen-ID: 675

NCBI Accession: NP_000050

UniProt: P51587

Pathways: DNA Reparatur, M Phase, Maintenance of Protein Location