CHEK1 Antikörper (pSer345)

Produktnummer ABIN317989

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CHEK1 in IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für CHEK1 Antikörper (pSer345) (ABIN317989)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CHEK1 Antikörper

Bindungsspezifität: pSer345

Spezifität: This antibody detects endogenous levels of Chk1 pSer345 protein.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10000approx. 1/20000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % Sodium Azide, approx. pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: CHK1

Hintergrund: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Molekulargewicht: approx. 56 kDa

Gen-ID: 1111

NCBI Accession: NP_001107593

UniProt: O14757

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur