CDC25C Antikörper (pSer216)

Produktnummer ABIN317983

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CDC25C in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CDC25C Antikörper (pSer216) (ABIN317983)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC25C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CDC25C Antikörper

Bindungsspezifität: pSer216

Spezifität: P-CDC25C (pSer216) antibody detects endogenous levels of p-CDC25C protein.

Aufreinigung: Affinity chromatography

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Andere Bezeichnung: CDC25C

Hintergrund: Cell cycle events are regulated by the sequential activation and deactivation of cyclin-dependent kinases (Cdks), including Cdk2 and Cdc2. Cdk2, in complexes with cyclin E and cyclin A, appears necessary for the onset and progression of DNA replication, while the Cdc2 kinase, in complexes with cyclin A or cyclin B, is required for the initiation of cell division. Wee 1 has been identified as a protein kinase that suppresses the entry into mitosis by mediating inhibiting tyrosine phosphorylation of Cdc2 p34. In contrast, members of the Cdc25 family of protein phosphatases function as mitotic activators by dephosphorylation of Cdc2 p34 on regulatory tyrosine and possibly threonine residues. The Cdc25 gene family consists of at least three members that share approximately 40 % identity in their most conserved carboxy-terminal sequences.Synonyms: Cell Division Cycle 25C, Dual specificity phosphatase Cdc25C, M-phase inducer phosphatase 3

Molekulargewicht: approx. 53 kDa

Gen-ID: 995

NCBI Accession: NP_001781

UniProt: P30307

Pathways: Zellzyklus, M Phase