CDC25C Antikörper (AA 175-473)

Produktnummer ABIN3042352

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CDC25C in WB, IF, FACS und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für CDC25C Antikörper (AA 175-473) (ABIN3042352)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC25C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-CDC25C Antikörper

Bindungsspezifität: AA 175-473

Verwendungszweck: Anti-Cdc25C Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: Human Cdc25C shares 78.2% amino acid (aa) sequence identity with mouse Cdc25C.

Produktmerkmale: Anti-Cdc25C Antibody. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cdc25C recombinant protein (Position: K175-P473). Human Cdc25C shares 78.2% amino acid (aa) sequence identity with mouse Cdc25C.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-CDC25C Antikörper (ABIN3042352)

Lin, Zhu, Sun, Yang, Sun, Li, Cui, Zheng, Yu: "Prostate cancer cell proliferation is suppressed by microRNA-3160-5p via targeting of F-box and WD repeat domain containing 8." in: Oncology letters, Vol. 15, Issue 6, pp. 9436-9442, (2018) (PubMed).

Ling, Lu, He, Xiao, Jiang, Su: "Diallyl disulfide selectively causes checkpoint kinase-1 mediated G2/M arrest in human MGC803 gastric cancer cell line." in: Oncology reports, Vol. 32, Issue 5, pp. 2274-82, (2014) (PubMed).

Details zu CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Andere Bezeichnung: CDC25C

Hintergrund:

Background: M-phase inducer phosphatase 3 is an enzyme that in humans is encoded by the CDC25C gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It s dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Gene Full Name: cell division cycle 25C

Molekulargewicht: 53 kDa

Gen-ID: 995

UniProt: P30307

Pathways: Zellzyklus, M Phase