Prothrombin Antikörper

Produktnummer ABIN317606

Der Kaninchen Polyklonal Anti-Prothrombin-Antikörper wurde für WB, IF und IHC (p) validiert. Er ist geeignet, Prothrombin in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für Prothrombin Antikörper (ABIN317606)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Prothrombin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Prothrombin Antikörper

Spezifität: This antibody detects endogenous levels of Prothrombin protein.(region surrounding Gly724)

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500-1/1000. ELISA: 1/10000-1/20000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Andere Bezeichnung: Prothrombin (F2)

Hintergrund: Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.Synonyms: Coagulation factor II

Molekulargewicht: approx. 70 kDa

Gen-ID: 2147

NCBI Accession: NP_000497

UniProt: P00734

Pathways: Komplementsystem, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling