VAMP2 Antikörper (N-Term)

Produktnummer ABIN317061

Dieses Anti-VAMP2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von VAMP2 in WB, IHC (p) und EIA. Geeignet für Human und Ratte.

Kurzübersicht für VAMP2 Antikörper (N-Term) (ABIN317061)

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VAMP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 3-00E-005

Produktdetails anti-VAMP2 Antikörper

Bindungsspezifität: AA 1-89, N-Term

Spezifität: This antibody recognizes specifically Synaptobrevin 2, but it also shows a low affinity to Synaptobrevin 1. Detects a band of approximately 14 kDa in rat brain lysates.

Produktmerkmale: Synonyms: VAMP2, Vesicle-associated membrane protein 2, SYB2

Aufreinigung: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Recombinant Human Synaptobrevin 2 (1-89 aa) purified from E. coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot (1/2,000-1/3,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This antibody was validated foruse in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubationwith the primary antibody, slides were incubated with biotinylated secondary antibody,followed by alkaline phosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Andere Bezeichnung: VAMP-2 / Synaptobrevin-2

Hintergrund: Synaptobrevin 2 (Syb2) also known as vesicle associated membrane protein-2 (VAMP2) is a membrane protein that belongs to the v-SNARE family of proteins, and is an essential component in neurotransmitter exocytosis from the presynaptic membrane. It is expressed on the synaptic vesicle, where it is bound by synaptophysin. Synaptophysin targets the vesicle to the presynaptic membrane and releases Syb2, which forms a stable complex with syntaxin and SNAP-25 (both expressed on the target membrane). Nerve cell depolarization and influx of calcium leads to vesicle fusion and neurotransmitter release into the synapse.Synonyms: SYB2, VAMP2, Vesicle-associated membrane protein 2

Gen-ID: 6844

UniProt: P63027

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport