SPHK1 Antikörper (Internal Region)

Produktnummer ABIN308402

Der Ziege Polyklonal anti-SPHK1 Antikörper wird verwendet zum Nachweis von SPHK1 in Proben von Human. Er wurde validiert für WB, ELISA und IP.

Kurzübersicht für SPHK1 Antikörper (Internal Region) (ABIN308402)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SPHK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Güteklasse: Verified

Produktdetails anti-SPHK1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: SPHK1

Sequenz: DVDLESEKYR RLGE

Spezifität: This antibody is expected to recognise all three reported isoforms (NP_068807.2, NP_892010.2, NP_001136073.1). Reported variants represent identical protein (NP_001136073.1, NP_001136074.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2, NP_892010.2, NP_001136073.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Andere Bezeichnung: SPHK1

Hintergrund: SPHK1, sphingosine kinase 1, SPHK, SK1, SKase

Gen-ID: 8877, 20698, 170897

NCBI Accession: NP_068807, NP_892010, NP_001136073

Pathways: VEGF Signaling