EC 2.7.1, SK 1, SPK 1, SPHK1, Gene name: SPHK1 or SPHK or SP,Sphingosine Kinase 1 (Sphk1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
Aufreinigung
Antigen Immunoaffiinity Purification
Immunogen
Rabbits were immunized with a synthetic peptide derived from the C-terminal of human Sphingosine Kinase 1
SPHK1
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Antibody can be used for Western blotting (1:400 dilution) and ELISA. Other applications not yet tested. Optimal concentration should be evaluated by serial dilutions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles