EGF Antikörper (AA 977-1029)

Produktnummer ABIN3044517

Dieses Anti-EGF-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von EGF in WB und ELISA. Geeignet für Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für EGF Antikörper (AA 977-1029) (ABIN3044517)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-EGF Antikörper

Bindungsspezifität: AA 977-1029

Verwendungszweck: Anti-EGF Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-EGF Antibody Picoband® (ABIN3044517). Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived mouse EGF recombinant protein (Position: N977-R1029). Mouse EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and rat EGF.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Mouse
1. Brissenden, J. E., Ullrich, A., Francke, U.Chromosomal mapping of loci for insulin-like growth factors I and II and for epidermal growth factor in man.Am. J. Hum. Genet.36: 133S-only, 1984. 2. Carpenter G, Cohen S (May 1990). "Epidermal growth factor". The Journal of Biological Chemistry 265 (14): 7709-12. 3. Herbst RS (2004). "Review of epidermal growth factor receptor biology". International Journal of Radiation Oncology, Biology, Physics 59 (2 Suppl): 21-6.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-EGF Antikörper (ABIN3044517)

Wang, Li, Fu, Li, Yang, Zhang, Zhu, Yang, Gu, Xing, Zhang: "Exemestane Attenuates Hepatic Fibrosis in Rats by Inhibiting Activation of Hepatic Stellate Cells and Promoting the Secretion of Interleukin 10." in: Journal of immunology research, Vol. 2017, pp. 3072745, (2018) (PubMed).

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: Egf

Hintergrund:

Synonyms: Pro-epidermal growth factor,EGF,Epidermal growth factor,Egf,

Tissue Specificity: Expressed in kidney, salivary gland, cerebrum and prostate. .

Background: EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Molekulargewicht: 134 kDa

Gen-ID: 13645

UniProt: P01132

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation