DIABLO Antikörper (AA 56-239)

Produktnummer ABIN3044515

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DIABLO in WB, IHC, ELISA, IF, FACS, IP und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.Es wurde in 1 Publikation zitiert.

Kurzübersicht für DIABLO Antikörper (AA 56-239) (ABIN3044515)

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DIABLO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-DIABLO Antikörper

Bindungsspezifität: AA 56-239

Verwendungszweck: Anti-Smac/Diablo Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: coli-derived human Smac,Diablo recombinant protein (Position: A56-D239). Human Smac,Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac,Diablo.

Produktmerkmale: Anti-Smac/Diablo Antibody. Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-DIABLO Antikörper (ABIN3044515)

You, Li, Jin, Zheng, Chen, Yang: "Genistein protects against Aβ25-35 induced apoptosis of PC12 cells through JNK signaling and modulation of Bcl-2 family messengers." in: BMC neuroscience, Vol. 18, Issue 1, pp. 12, (2017) (PubMed).

Details zu DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Andere Bezeichnung: DIABLO

Hintergrund:

Background: Diablo homolog  (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO  ( IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis.  A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: diablo IAP-binding mitochondrial protein

Molekulargewicht: 20 kDa

Gen-ID: 56616

UniProt: Q9NR28

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity