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DIABLO Antikörper (AA 56-239)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DIABLO in WB, ELISA, IHC, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.
Produktnummer ABIN3044515

Kurzübersicht für DIABLO Antikörper (AA 56-239) (ABIN3044515)

Target

Alle DIABLO Antikörper anzeigen
DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reaktivität

  • 61
  • 28
  • 27
  • 3
  • 2
  • 2
  • 2
Human, Maus, Ratte

Wirt

  • 55
  • 8
  • 1
Kaninchen

Klonalität

  • 45
  • 19
Polyklonal

Konjugat

  • 39
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
Dieser DIABLO Antikörper ist unkonjugiert

Applikation

  • 39
  • 19
  • 17
  • 7
  • 6
  • 6
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 11
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 56-239

    Verwendungszweck

    Anti-Smac/Diablo Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-Smac/Diablo Antibody Picoband® (ABIN3044515). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. "Entrez Gene: DIABLO diablo homolog (Drosophila)". 2. McNeish IA, Bell S, McKay T, Tenev T, Marani M, Lemoine NR (Jun 2003). "Expression of Smac/DIABLO in ovarian carcinoma cells induces apoptosis via a caspase-9-mediated pathway". Experimental Cell Research 286 (2): 186-98. 3. Yu J, Wang P, Ming L, Wood MA, Zhang L (Jun 2007). "SMAC/Diablo mediates the proapoptotic function of PUMA by regulating PUMA-induced mitochondrial events". Oncogene 26 (29): 4189-98.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • You, Li, Jin, Zheng, Chen, Yang: "Genistein protects against Aβ25-35 induced apoptosis of PC12 cells through JNK signaling and modulation of Bcl-2 family messengers." in: BMC neuroscience, Vol. 18, Issue 1, pp. 12, (2017) (PubMed).

  • Target

    DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

    Andere Bezeichnung

    DIABLO

    Hintergrund

    Synonyms: Diablo homolog, mitochondrial; IAP-binding protein with low pI;Second mitochondria-derived activator of caspase;Smac;DIABLO;SMAC;

    Tissue Specificity: Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. .

    Background: Diablo homolog  (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO  ( IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis.  A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

    Molekulargewicht

    25 kDa

    Gen-ID

    56616

    Pathways

    Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
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