CD25 Antikörper (N-Term)

Produktnummer ABIN3044139

Dieses Anti-CD25-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CD25 in WB und IHC. Geeignet für Human.

Kurzübersicht für CD25 Antikörper (N-Term) (ABIN3044139)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-CD25 Antikörper

Bindungsspezifität: AA 39-57, N-Term

Verwendungszweck: Anti-IL2RA Antibody Picoband®

Sequenz: MAYKEGTMLN CECKRGFRR

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-IL2RA Antibody (ABIN3044139). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD25/IL-2sR Alpha.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. Ansano, M., Toda, M., Sakaguchi, N., Sakaguchi, S. Autoimmune disease as a consequence of developmental abnormality of a T cell subpopulation. J. Exp. Med. 184: 387-396, 1996. 2. Cosman, D., Cerretti, D. P., Larsen, A., Park, L., March, C., Dower, S., Gillis, S., Urdal, D. Cloning, sequence and expression of human interleukin-2 receptor. Nature 312: 768-771, 1984. 3. Hori, S., Nomura, T., Sakaguchi, S. Control of regulatory T cell development by the transcription factor Foxp3. Science 299: 1057-1061, 2003.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: IL2RA

Hintergrund:

Synonyms: Interleukin-2 receptor subunit alpha,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,CD25,IL2RA,

Tissue Specificity: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney. .

Background: IL2RA (Interleukin 2 receptor, alpha), also called IL2R, IL2R, Alpha Chain or CD25, is a protein that in humans is encoded by the IL2RA gene.The IL2RA gene is mapped on 10p15.1. IL2RA is composed of strand-swapped "sushi-like" domains, unlike the classical cytokine receptor fold. As a result of this domain swap, IL2RA uses a composite surface to dock into a groove on IL2 that also serves as a binding site for antagonist drugs.Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Infection by the protozoan Trypanosoma cruzi causes Chagas disease, characterized by a reduction in the amount of IL2RA expressed on the surface of immune cells. This leads to chronic immune suppression, becoming increasingly severe over the course of many years and ultimately resulting in death if left untreated.

Sequence Similarities: Contains 2 Sushi (CCP/SCR) domains.

Molekulargewicht: 65 kDa

UniProt: P01589

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation