GLUT4 Antikörper (AA 333-509)

Produktnummer ABIN3043929

Produktdetails anti-GLUT4 Antikörper

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Bindungsspezifität: AA 333-509

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 4(SLC2A4) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 4(SLC2A4) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: solute carrier family 2 (facilitated glucose transporter), member 4
Protein Name: Solute carrier family 2, facilitated glucose transporter member 4

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GLUT4 recombinant protein (Position: N333-D509). Human GLUT4 shares 97% amino acid (aa) sequence identity with mouse GLUT4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, The detection limit for GLUT4 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-GLUT4 Antikörper (ABIN3043929)

Wei, An, Jin, Li, Xu: "Inhibition of insulin resistance by PGE1 via autophagy-dependent FGF21 pathway in diabetic nephropathy." in: Scientific reports, Vol. 8, Issue 1, pp. 9, (2018) (PubMed).

Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." in: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Huang, Zhang, Sun, Xu, Yi: "Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 9651592, (2016) (PubMed).

Zhou, Wu, Chen, Wang, Wang: "AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid." in: Nutrition & metabolism, Vol. 12, pp. 10, (2015) (PubMed).

Details zu GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Andere Bezeichnung: SLC2A4 (SLC2A4 Produkte)

Synonyme: GLUT4 antikoerper, Glut-4 antikoerper, Glut4 antikoerper, glut4 antikoerper, slc2a4 antikoerper, SLC2A4 antikoerper, solute carrier family 2 member 4 antikoerper, solute carrier family 2 (facilitated glucose transporter), member 4 antikoerper, solute carrier family 2 (facilitated glucose transporter), member 4 L homeolog antikoerper, SLC2A4 antikoerper, Slc2a4 antikoerper, GLUT4 antikoerper, slc2a4.L antikoerper

Hintergrund: GLUT4, also known as SLC2A4 or solute carrier family 2 (facilitated glucose transporter) member 4, is a protein that in humans is encoded by the GLUT4 gene. It is mapped to 17p13.1. This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

Synonyms: insulin-responsive antibody|Glucose transporter GLUT 4 antibody|Glucose transporter type 4 antibody|Glucose transporter type 4 insulin responsive antibody|GLUT 4 antibody|GLUT-4 antibody|GLUT4 antibody|GTR4_HUMAN antibody|kug antibody|SLC 2A4 antibody|SLC2A4 antibody|solute carrier family 2 (facilitated glucose transporter) member 4 antibody|Solute carrier family 2, facilitated glucose transporter member 4 antibody

Gen-ID: 6517

UniProt: P14672

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effekt